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005 20260702004755.0
008 260701s2023 ag |||||||||||||||||spa
100 1 _aKamalak
_9199407
700 1 _aKamalak, Aliye
_9199408
700 1 _aTaghizadehghalehjoughi, Ali
_9199409
700 1 _aAlp Nanci, Kemal
_9199410
245 1 0 _aEfectos citotóxicos y biológicos de los compuestos de relleno masivo en células neuronales corticales de rata
246 1 3 _aCytotoxic and biological effects of bulk fill composites on rat cortical neuron cells
264 1 _c2023
300 _a12 páginas
520 _aThe aim of this study was to investigate potential cellular responses and biological effects of new generation dental composites on cortical neuron cells in two different exposure times. The study group included five different bulk-fill flow able composites; Surefil SDR Flow, X-tra Base Flow, Venus Bulk Flow, Filtek Bulk Flow and Tetric-Evo Flow. They were filled in Teflon molds (Height: 4 mm, Width: 6 mm) and irradiated for 20 s. Cortical neuron cells were inoculated into 24-well plates. After 80% of the wells were coated, the 3 µm membrane was inserted and dental filling materials were added. The experiment was continued for 24 and 72 h. Cell viability measured by MTT assay test, total antioxidant and total oxidant status were examined using real assay diagnostic kits. The patterns of cell death (apoptosis) were analyzed using annexin V-FITC staining with flow cytometry. ?-defensins were quantitatively assessed by RT-PCR. IL-6, IL-8 and IL-10 cytokines were measured from the supernatants. All composites significantly affected analyses parameters during the exposure durations. Our data provide evidence that all dental materials tested are cytotoxic in acute phase and these effects are induced cellular death after different exposure periods. Significant cytotoxicity was detected in TE, XB, SS, FBF and VBF groups at 24 and 72 h, respectively.
650 4 _aApoptosis
_9136434
650 4 _aViabilidad Celular
_9197904
650 4 _aCitotoxicidad
_9168340
650 4 _aCapacidad Antioxidante Total
_9199411
650 4 _aEstado Oxidante Total
_9199412
773 0 _tOdontology
_x1618-1247
_gv. 106 n. 4 (2018)
_w257190
942 _cART
035 _a(ODN)65677
001 266521
999 _c266521
_d266521
040 _aAR-ReUNN
_bspa
_cAR-ReUNN
_eaacr2