000			03192nab a2200337 a 4500
003			AR-ReUNN
005			20260702004709.0
008			260701s2021 ag |||||||||||||||||eng
041	0		_aeng
100	1		_aRomero Castro, Norma Samanta _9196992
700	1		_aVázquez Villamar, Mirna _9196993
700	1		_aMuñoz Valle, José Francisco _9196994
700	1		_aSerna Radilla, Víctor Othón _9196995
700	1		_aGarcía Arellano, Samuel _9196996
700	1		_aCastro Alarcón, Natividad _9196997
245	1	0	_aRelación entre los niveles de TNF-a, MMP-8 y MMP-9 en el líquido crevicular gingival y la microbiota subgingival en la enfermedad periodontal.
246	1	3	_aRelationship between Tnf-A, mmp-8, and mmp-9 levels in gingival crevicular fluid and the subgingival microbiota in periodontal disease
264		1	_c2021
300			_a9 páginas
520			_aPeriodontitis is modulated by a complex dysbiotic microbiota, these species stimulate upward the production of pro-inflammatory cytokines such as TNF-a, which, in turn, upregulates the production of bone resorption molecules. Enzymes such as MMP-8 and 9 have been associated with the destructive disease. This study evaluated the composition of periodontal microbiota with the checkerboard hybridization technique and its correlation with TNF-a, MMP-8, and MMP-9 evaluated with ELISA, of 80 patients (45 healthy, and 35 with chronic periodontitis). The frequency of the 18 species evaluated was higher in patients with bone loss compared with control group. TNF-a in gingival crevicular fluid was significantly higher in bone loss group (p?<?0.01); MMP-8 (p?=?0.34) by MMP-9 (p?<?0.05) in bone loss group obtained lower values than in control group. Positive correlation of TNF-a was obtained with Aggregatibacter actinomycetemcomitans (rho?=?0.38; p?<?0.01), Fusobacterium nucleatum (rho?=?0.25; p?<?0.05) and Porphyromonas gingivalis (rho?=?0.26; p?<?0.05); negative correlation of MMP-8 with A. actinomycetemcomitans (rho?=?0.26; p?<?0.01), Capnocytophaga sputigena (rho?=?0.33; p?<?0.01), and F. nucleatum (rho?=?0.21; p?<?0.05); also negative correlation of MMP-9 with F. nucleatum (rho?=?0.23; p?<?0.05), P. gingivalis (rho?=?0.23; p?<?0.05), and Tannerella forsythia (rho?=?0.26; p?<?0.01). TNF-a increased due to the increase in each count of A. actinomycetemcomitans (ß?=?0.57; p?=?0.00). The presence of A. actinomycetemcomitans (ß?=?1.88; p?=?0.00), Campylobacter rectus (ß?=?0.78; p?=?0.01), F. nucleatum (ß?=?0.65; p?=?0.04), and P. gingivalis (ß?=?0.65; p?=?0.04) significantly increases TNF-a levels. TNF-a in gingival crevicular fluid, despite the minimal amounts collected, is a good biomarker of periodontal disease; since levels of TNF-a increases with the increase of the most harmful species to the periodontium.
650		4	_aMicrobiología _96031
650		4	_aFluido Crevicular _9191796
650		4	_aFluido Gingival _9171636
650		4	_aEnfermedades Periodontales _910289
650		4	_aCitocinas _9167746
650		4	_aMetaloproteinas de la Matriz _9196998
773	0		_tOdontology _x1618-1247 _gv. 108 n. 1 (2020) _w257190
942			_cART
035			_a(ODN)64303
001			265436
999			_c265436 _d265436
040			_aAR-ReUNN _bspa _cAR-ReUNN _eaacr2