000			01978nab a2200241 a 4500
003			AR-ReUNN
005			20260702003425.0
008			260630s2007 ag |||||||||||||||||spa
100	1		_aBertoni, María Delia _9155551
700	1		_aAdler, Mónica T. _9155216
700	1		_aMaier, Marta S. _9157604
245	1	0	_aCultivo "in vitro" de micobiontes de Parmotrema eciliatum y Flavoparmelia exornata (Parmeliaceae, ascomycota liquenizados) con producción de trigliceridos / M. D. Bertoni; M. T. Adler; M. S. Maier
264		1	_c2007
300			_a5 páginas
500			_afot. byn.
520			_aIn vitro culture of mycobionts of Parmotrema eciliatum and Flavoparmelia exornata (Parmeliaceae, lichenized Ascomycota) with production of triglycerides. The mycobionts of Parmotrema eciliatum (Nyl.) Hale and Flavoparmeilia exormata (Zahlbr.) Hale have been cultured axenically on solid medium (malt extract-yeast extract-sucrose-agar) starting from ascospores. Growth was extremely slow in both species, whose myceliarached only 6-10 mm diam. after 10 months. The colonies were very compact, with fungal tissue constiuting mostly a paraplectenchymatous lamina with revolute margins and poor development of arial hyphae. Chemical analysis of the mycobionts by thin layer chromatography after 4, 7 and 10 months of growth did not detect accumulation of the major secondary metabolites characteristic of the natural thalli, namel atranorin and stictic acid in Parmotema eciliatum, and usnic and malonprotecetariacids in Flavoparmelia exornata, compounds of the acetate-polymalonate pathway. Nuclear magnetic resonance spectra taken after 10 months revealed mainly the accumulation of triglycerides (lipids of primary metabolism) by the mycobionts.
650		4	_aLiquenes _963181
650		4	_aVitroplantas _9115480
773	0		_tBoletín de la Sociedad Argentina de Botánica _x0373-580X _gv. 34 n. 3-4 (2000) _w238707
942			_cART
035			_a(IBONE)44518
001			242258
999			_c242258 _d242258
040			_aAR-ReUNN _bspa _cAR-ReUNN _eaacr2