02884nab a2200349 a 4500 AR-ReUNN 20260702004712.0 260701s2021 ag |||||||||||||||||eng eng Johannessen, Anne Christine 197172 Neppelberg, Evelyn 197173 Liang, Xiao 197174 Das, Ridhima 197175 Kitajima, Kayoto 197176 Igarashi, Masaru 197177 Costea, Daniela Elena 197178 Igarashi, Masaru 197177 Aislamiento y caracterización de células derivadas de restos epiteliales humanos de Malassez Isolation and characterization of cells derived from human epithelial rests of malassez 2021 10 páginas The epithelial rests of Malassez (ERMs) might represent a valuable source of oral epithelial cells with stem cell properties. The purpose of this study was to isolate and characterize cells derived from human ERM, and compare them with cells derived from matched normal oral mucosa (NOM). Matched tissue specimens of the periodontal ligament of extracted tooth and NOM were collected. Cells were isolated in culture, then characterized by immunohistochemistry and flow cytometry for expression of pancytokeratin, ESA, PDGFRB, CD31 and CD44. 3D organotypic cultures were constructed by growing epithelial cells on top of fibroblast-populated collagen gels. Both ERM and NOM-isolated cells expressed the markers of epithelial lineage (ESA and pancytokeratin), and to some extent PDGFR, an indicator of a more mesenchymal phenotype, but not the endothelial cell marker CD31. Cells with epithelial morphology were isolated from periodontium of cervical, middle and apical parts of the root, but contained a significantly lower percentage of ESA and pancytokeratin-positive cells than when isolating cells from NOM (p?<?0.001). ERM cells expressed a significantly higher percentage of the stem cell-related molecule CD44 (cervical 92.93?±?0.25%, middle 93.8?±?0.26%, apical 94.36?±?0.41%) than cells isolated from NOM (27.8?±?1.47%, p?<?0.001). When grown in 3D organotypic cultures and in collagen gels, ERM cells formed a less differentiated epithelium than NOM cells, but expressing pancytokeratin and vimentin. In conclusion, epithelial cells could be isolated from human periodontium and grown in culture; their in vitro characterization indicates that they have a less differentiated phenotype compared with cells derived from normal oral epithelium. Restos de Malassez 197179 Ligamento Periodontal 166272 Inmunohistoquimica 125622 Humano 122622 Mucosa Oral 167435 Odontology 1618-1247 v. 107 n. 3 (2019) 257190 ART (ODN)64362 265476 265476 265476 AR-ReUNN spa AR-ReUNN aacr2