02773nab a2200361 a 4500003000900000005001700009008004100026041000800067100002200075700002500097700003100122700002800153700002600181700002800207700003000235700002900265245010600294246009300400264000900493300001600502520162300518650002702141650003202168650002702200650002502227650002102252773005402273942000802327035001502335001000702350999001902357040003502376AR-ReUNN20260702004710.0260701s2021    ag |||||||||||||||||eng  0 aeng1 aIto, Yuki91970311 aIto, Takashi91970321 aYamashiro, Keisuke91970331 aMineshiba, Fumi91970341 aHirai, Kimito91970351 aOmori, Kazuhiro91970361 aYamamoto, Tadashi91970371 aTakashiba, Shogo919580710aEfectos antimicrobianos y antibiofilm del ácido abiético sobre Streptococcus mutans cariogénico13aAntimicrobial and antibiofilm effects of abietic acid on cariogenic streptococcus mutans 1c2021  a9 páginas  aDental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection. 4aAcido Abietico9197038 4aStreptococcus Mutans910290 4aAntimicrobiano9195431 4aBiofilm Oral9197039 4aTerpenos91970400 tOdontologyx1618-1247gv. 108 n. 1 (2020)w257190  cART  a(ODN)64309265441  c265441d265441  aAR-ReUNNbspacAR-ReUNNeaacr2