03107nab a2200325 a 4500003000900000005001700009008004100026041000800067100003300075700003000108700003600138700003600174700003000210700003200240245015100272246013900423264000900562300001600571520194300587650002002530650002202550650002002572650003102592650001402623650003302637773005402670035001502724001000702739040003502746AR-ReUNN20260702004709.0260701s2021    ag |||||||||||||||||eng  0 aeng1 aRomero Castro, Norma Samanta1 aVázquez Villamar, Mirna1 aMuñoz Valle, José Francisco1 aSerna Radilla, Víctor Othón1 aGarcía Arellano, Samuel1 aCastro Alarcón, Natividad10aRelación entre los niveles de TNF-a, MMP-8 y MMP-9 en el líquido crevicular gingival y la microbiota subgingival en la enfermedad periodontal.13aRelationship between Tnf-A, mmp-8, and mmp-9 levels in gingival crevicular fluid and the subgingival microbiota in periodontal disease 1c2021  a9 páginas  aPeriodontitis is modulated by a complex dysbiotic microbiota, these species stimulate upward the production of pro-inflammatory cytokines such as TNF-a, which, in turn, upregulates the production of bone resorption molecules. Enzymes such as MMP-8 and 9 have been associated with the destructive disease. This study evaluated the composition of periodontal microbiota with the checkerboard hybridization technique and its correlation with TNF-a, MMP-8, and MMP-9 evaluated with ELISA, of 80 patients (45 healthy, and 35 with chronic periodontitis). The frequency of the 18 species evaluated was higher in patients with bone loss compared with control group. TNF-a in gingival crevicular fluid was significantly higher in bone loss group (p?<?0.01); MMP-8 (p?=?0.34) by MMP-9 (p?<?0.05) in bone loss group obtained lower values than in control group. Positive correlation of TNF-a was obtained with Aggregatibacter actinomycetemcomitans (rho?=?0.38; p?<?0.01), Fusobacterium nucleatum (rho?=?0.25; p?<?0.05) and Porphyromonas gingivalis (rho?=?0.26; p?<?0.05); negative correlation of MMP-8 with A. actinomycetemcomitans (rho?=?0.26; p?<?0.01), Capnocytophaga sputigena (rho?=?0.33; p?<?0.01), and F. nucleatum (rho?=?0.21; p?<?0.05); also negative correlation of MMP-9 with F. nucleatum (rho?=?0.23; p?<?0.05), P. gingivalis (rho?=?0.23; p?<?0.05), and Tannerella forsythia (rho?=?0.26; p?<?0.01). TNF-a increased due to the increase in each count of A. actinomycetemcomitans (ß?=?0.57; p?=?0.00). The presence of A. actinomycetemcomitans (ß?=?1.88; p?=?0.00), Campylobacter rectus (ß?=?0.78; p?=?0.01), F. nucleatum (ß?=?0.65; p?=?0.04), and P. gingivalis (ß?=?0.65; p?=?0.04) significantly increases TNF-a levels. TNF-a in gingival crevicular fluid, despite the minimal amounts collected, is a good biomarker of periodontal disease; since levels of TNF-a increases with the increase of the most harmful species to the periodontium. 4aMicrobiología 4aFluido Crevicular 4aFluido Gingival 4aEnfermedades Periodontales 4aCitocinas 4aMetaloproteinas de la Matriz0 tOdontologyx1618-1247gv. 108 n. 1 (2020)w257190  a(ODN)64303265436  aAR-ReUNNbspacAR-ReUNNeaacr2