03029nab a2200445 a 4500003000900000005001700009008004100026041000800067100002600075700002600101700002700127700002700154700002600181700003000207700002300237700002600260700003100286700003200317700002500349700002900374245022000403246020100623264000900824300001600833520134800849650003402197650003002231650002202261650003202283650004102315650002402356650004002380650002502420773005402445942000802499035001502507001000702522999001902529040003502548AR-ReUNN20260702004709.0260701s2021    ag |||||||||||||||||eng  0 aeng1 aToraya, Seiko91969791 aUehara, Osamu91969801 aHiraki, Daichi91969811 aHarada, Fumiya91969821 aNeopane, Puja91969831 aMorikawa, Tetsuro91969841 aTakai, Rie91969851 aYoshida, Koki91969861 aMatsuoka, Hirofumi91969871 aKitaichi, Nobuyoshi91969881 aChiba, Itsuo91969891 aAbiko, Yoshihiro919699013aLa curcumina inhibe la expresión de mediadores proinflamatorios y MMP-9 en células epiteliales gingivales estimuladas durante un período prolongado con lipopolisacáridos derivados de Porphyromonas gingivalis13aCurcumin inhibits the expression of proinflammatory mediators and mmp-9 in gingival epithelial cells stimulated for a proloned period whit lipopolysaccharides derived from porphyromonas gingivalis 1c2021  a9 páginas  aCurcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-a, IL-1ß, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-?B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-a, IL-1ß, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1ß, IL-6, and MMP-9 were decreased in the culture supernatants. NF-?B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-?B activation. 4aFracturas Complicadas9168255 4aLipopolisacaridos9144642 4aCurcumina9196991 4aCelulas Epiteliales9124034 4aPatología Bucomaxilofacial9190780 4aInhibición915880 4aMediadores Proinflamatorios9174273 4aEstimulación951220 tOdontologyx1618-1247gv. 108 n. 1 (2020)w257190  cART  a(ODN)64302265435  c265435d265435  aAR-ReUNNbspacAR-ReUNNeaacr2