TY  - SER
AU  - Toraya,Seiko
AU  - Uehara,Osamu
AU  - Hiraki,Daichi
AU  - Harada,Fumiya
AU  - Neopane,Puja
AU  - Morikawa,Tetsuro
AU  - Takai,Rie
AU  - Yoshida,Koki
AU  - Matsuoka,Hirofumi
AU  - Kitaichi,Nobuyoshi
AU  - Chiba,Itsuo
AU  - Abiko,Yoshihiro
TI  - La curcumina inhibe la expresión de mediadores proinflamatorios y MMP-9 en células epiteliales gingivales estimuladas durante un período prolongado con lipopolisacáridos derivados de Porphyromonas gingivalis
PY  - 2021///
KW  - Fracturas Complicadas
KW  - Lipopolisacaridos
KW  - Curcumina
KW  - Celulas Epiteliales
KW  - Patología Bucomaxilofacial
KW  - Inhibición
KW  - Mediadores Proinflamatorios
KW  - Estimulación
N2  - Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-a, IL-1ß, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-?B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-a, IL-1ß, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1ß, IL-6, and MMP-9 were decreased in the culture supernatants. NF-?B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-?B activation
ER  -