La Toraya, Seiko creator Uehara, Osamu Hiraki, Daichi Harada, Fumiya Neopane, Puja Morikawa, Tetsuro Takai, Rie Yoshida, Koki Matsuoka, Hirofumi Kitaichi, Nobuyoshi Chiba, Itsuo Abiko, Yoshihiro text ag 2021 continuing eng 9 páginas Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-a, IL-1ß, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-?B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-a, IL-1ß, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1ß, IL-6, and MMP-9 were decreased in the culture supernatants. NF-?B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-?B activation. Fracturas Complicadas Lipopolisacaridos Curcumina Celulas Epiteliales Patología Bucomaxilofacial Inhibición Mediadores Proinflamatorios Estimulación 1618-1247 257190 v. 108 n. 1 (2020) AR-ReUNN 260701 20260702004709.0 265435 spa