02971nab a2200433 a 4500 AR-ReUNN 20260702004709.0 260701s2021 ag |||||||||||||||||eng eng Toraya, Seiko 196979 Uehara, Osamu 196980 Hiraki, Daichi 196981 Harada, Fumiya 196982 Neopane, Puja 196983 Morikawa, Tetsuro 196984 Takai, Rie 196985 Yoshida, Koki 196986 Matsuoka, Hirofumi 196987 Kitaichi, Nobuyoshi 196988 Chiba, Itsuo 196989 Abiko, Yoshihiro 196990 La curcumina inhibe la expresión de mediadores proinflamatorios y MMP-9 en células epiteliales gingivales estimuladas durante un período prolongado con lipopolisacáridos derivados de Porphyromonas gingivalis Curcumin inhibits the expression of proinflammatory mediators and mmp-9 in gingival epithelial cells stimulated for a proloned period whit lipopolysaccharides derived from porphyromonas gingivalis 2021 9 páginas Curcumin, a yellow phytochemical found in the rhizomes of Curcuma longa, has various biological effects, including anti-oxidant and anti-inflammatory activities. In the present study, we examined the effect of curcumin on the expression of inflammatory cytokines in human gingival epithelial progenitor cells (HGEPs) stimulated for a prolonged period with lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The cells were alternately cultured with LPS and/or curcumin every 3 days for 18 days. The expression levels of TNF-a, IL-1ß, IL-6, TIMP-1, and MMP-9 in the HGEPs were evaluated by quantitative real-time polymerase chain reaction. Enzyme-linked immunosorbent assay was used to measure the concentrations of these five proteins in the supernatant and nuclear factor (NF)-?B in the nuclear extracts. Curcumin inhibited the mRNA expression levels of TNF-a, IL-1ß, IL-6, and MMP-9 in HGEPs treated with curcumin over a prolonged period. Similarly, the expression levels of IL-1ß, IL-6, and MMP-9 were decreased in the culture supernatants. NF-?B activity was also inhibited in the cells cultured with curcumin. In conclusion, these findings indicate that curcumin inhibits the expression of inflammatory cytokines and MMP-9 in primary gingival epithelial cells stimulated with P. gingivalis-derived LPS via NF-?B activation. Fracturas Complicadas 168255 Lipopolisacaridos 144642 Curcumina 196991 Celulas Epiteliales 124034 Patología Bucomaxilofacial 190780 Inhibición 15880 Mediadores Proinflamatorios 174273 Estimulación 5122 Odontology 1618-1247 v. 108 n. 1 (2020) 257190 ART (ODN)64302 265435 265435 265435 AR-ReUNN spa AR-ReUNN aacr2