02816nab a2200325 a 4500 AR-ReUNN 20260702002306.0 260630s2007 ag |||||||||||||||||eng eng Coppo, José Antonio 117924 Mussart, Norma B. 9402 Fioranelli, Santiago a. 125730 Physiological variation of enzymatic activities in blood of bullfrog, Rana catesbeiana Shaw, 1802 / J. A. Coppo ; N. B. Mussart ; S. A. Fioranelli 2007 6 páginas Abstract: The aim of this study was to determine plasma enzymogram reference values in bullfrog, Rana catesbeiana, to correlate them with physiological modifications attributable to sex, age, weight, climate, and breeding and feeding systems. For this purpose, three hundred and two healthy animals (from 9 to 21 months- old, both sexes) were used. Reference intervals for alkaline phosphatase, ALP (157±32 IU/L), alanine aminotransferase, ALT (12.4±2.6 IU/L), aspartate aminotransferase, AST (48.1±9.3 IU/L), gammaglutamyl transferase, GGT (9.2±1.6 IU/L), lactate dehydrogenase, LDH (117±22 IU/L), butyryl cholinesterase, CHE (168±32 IU/L) and creatine phosphokinase, CPK (432±85 IU/L), were obtained. Growth showed a correlation to weight increase (r =0.82, p =0.02). Significant lineal association between the age increment and ALP decrease (9 months = 196 versus 21 months = 102 IU/L), as well as the increases of CHE (126 versus 226 IU/L) and CPK (280 versus 572 IU/L), were registered. ALP and CPK were higher in males than females (p<0.05). Except GGT, enzymatic activities were higher in winter than on the other seasons. ALP, AST, GGT and LDH values were significantly higher when water covered 90% of the tank floor and food was administered as floating pellets. On the contrary, values were low when water covered only 25% of the tank and pellets were given on the floor. The highest values on hepatic strain marker enzymes (ALP, ALT, AST, GGT and LDH) were registered in frogs which have eaten bovine lung, with or without balanced pellets, and the lowest ones were verified on animals fed naturally in a lagoon. The usefulness of enzymogram to evaluate sanitary, metabolic, and nutritional state is proposed, while its application as suitable tool to improve frog meat production is emphasized. Rana Catesbeiana 83046 Rana 9251 Plasma 80296 Physiology 125964 Lithobates Catesbeianus 125806 Cria de Ranas 62125 Amphibia 125870 Anfibios 9696 Anuros 118589 Revista Veterinaria 1668-4834 v. 12/13 n. 1-2 (2002) 217547 ART (AGROP)41405 217840 217840 217840 AR-ReUNN spa AR-ReUNN aacr2